goat polyclonal ccl20  (R&D Systems)

Journal: Cytokine

Article Title: An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium

doi: 10.1016/j.cyto.2017.05.023

Figure Lengend Snippet: Chemokines present at VWF positive vessels in RA synovia.

Article Snippet: Briefly, sections were blocked then incubated for 1 h in the primary antibodies, the working concentrations used were: anti-human mouse monoclonal CCL2 (10 μg/ml; MAB2791), mouse monoclonal CCL3 (10 μg/ml; MAB270), goat polyclonal CCL4 (10 μg/ml; AF-271-NA), goat polyclonal CCL5 (15 μg/ml; AF-278-NA), mouse monoclonal CCL11 (15 μg/ml; MAB320), goat polyclonal CCL16 (15 μg/ml; AF802), goat polyclonal CCL17 (10 μg/ml; AF364), mouse monoclonal CCL19 (20 μg/ml; MAB361), goat polyclonal CCL20 (10 μg/ml; AF360), goat polyclonal CCL21(15 μg/ml; AF366), goat polyclonal CCL24 (15 μg/ml; AF343), goat polyclonal CCL26 (10 μg/ml; AF653), mouse monoclonal CCL27 (20 μg/ml; MAB367), (all R&D Systems, UK), goat polyclonal CCL8 (2 μg/ml; Sc-1307), goat polyclonal CCL13 (4 μg/ml; Sc-9655), mouse monoclonal CCL14 (2 μg/ml; Sc-28388), goat polyclonal CCL18 (4 μg/ml; Sc-9781), goat polyclonal CCL22 (4 μg/ml; Sc-12285), goat polyclonal CCL23 (4 μg/ml; Sc-12263), goat polyclonal CCL25 (2 μg/ml; Sc-12277) and goat polyclonal CCL28 (4 μg/ml; Sc-27339) (all SantaCruz Biotechnology Inc UK), mouse monoclonal CCL1 (2.5 μg/ml; LS-C4342) and rabbit polyclonal CCL7 (2.5 μg/ml; LS-B930) (LifeSpan Biosciences, UK), rabbit polyclonal CCL10 (4 μg/ml; Orb13568), rabbit polyclonal CCL12 (2 μg/ml; Orb132384), rabbit polyclonal CCL15 (4 μg/ml; Sc-28388) (Biorbyte, UK), rabbit anti-human von Willebrand Factor (VWF) (3 μg/ml; A0082) and mouse anti-human VWF (4 μg/ml; M0616) (Dakocytomation, UK).

Techniques:

Journal: Oncology reports

Article Title: miR-21 and its target gene CCL20 are both highly overexpressed in the microenvironment of colorectal tumors: significance of their regulation.

doi: 10.3892/or.2013.2580

Figure Lengend Snippet: Figure 1. CCL20 mRNA expression in CRC of different clinical charac teristics as determined by q-RT-PCR. q-RT-PCR data are expressed as means ± standard error of the mean (SEM), *P<0.05, n=46. Fold increase >1 indicates CCL20 overexpression in CRC tissues relative to the unaffected adjacent tissues, respectively. (A) Mean data of all investigated patients. (B) Mean patient data according to T-classification. (C) Mean patient data according to clinical staging. (D) Mean patient data according to differentia tion states.

Article Snippet: Overnight incubation of slides with primary goat polyclonal anti-human CCL20 antibody (15 μg/ml, AF360; R&D Systems) was followed by incubation with secondary biotinylated rabbit anti-goat IgG antibody and the avidin-biotin-peroxidase reaction (Vector Laboratories).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Over Expression

Journal: Oncology reports

Article Title: miR-21 and its target gene CCL20 are both highly overexpressed in the microenvironment of colorectal tumors: significance of their regulation.

doi: 10.3892/or.2013.2580

Figure Lengend Snippet: Figure 2. CCL20 protein expression in CRC of different clinical charac teristics as determined by ELISA assays. ELISA results are presented as absolute values of pg/ml chemokine ligand per mg total protein in CRC tis sues and unaffected adjacent tissues, respectively. The data are expressed as means ± standard error of the mean (SEM), *P<0.05, n=46. (A) Mean data of all investigated patients. (B) Mean patient data according to T-classification. (C) Mean patient data according to clinical staging. (D) Mean patient data according to differentiation states.

Article Snippet: Overnight incubation of slides with primary goat polyclonal anti-human CCL20 antibody (15 μg/ml, AF360; R&D Systems) was followed by incubation with secondary biotinylated rabbit anti-goat IgG antibody and the avidin-biotin-peroxidase reaction (Vector Laboratories).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Oncology reports

Article Title: miR-21 and its target gene CCL20 are both highly overexpressed in the microenvironment of colorectal tumors: significance of their regulation.

doi: 10.3892/or.2013.2580

Figure Lengend Snippet: Figure 4. Localization of CCL20 protein expression in CRC. Representative specimens of the normal mucosa as well as of the colorectal adenocarci nomas were probed with antibodies against CCL20 (15 µg/ml; ABC method). (A) In unaffected colorectal tissues, immunoreactivity against CCL20 was detected in the perinuclear regions of mucosal epithelial cells (arrowheads) and mesenchymal cells (arrows). (B) In the colorectal tissue specimens, immunoreactive signals against CCL20 were predominantly detected in the mesenchymal portions (arrows) while no anti-CCL20 signals were observed in adenocarcinoma cells. (C) One specimen revealed immunoreactivity against CCL20 in both adenocarcinoma cells (black asterisks) and mesen chymal elements (black arrows).

Article Snippet: Overnight incubation of slides with primary goat polyclonal anti-human CCL20 antibody (15 μg/ml, AF360; R&D Systems) was followed by incubation with secondary biotinylated rabbit anti-goat IgG antibody and the avidin-biotin-peroxidase reaction (Vector Laboratories).

Techniques: Expressing